Journal: Molecular Therapy Oncolytics

Article Title: Resveratrol Inhibits the Tumorigenesis of Follicular Thyroid Cancer via ST6GAL2-Regulated Activation of the Hippo Signaling Pathway

doi: 10.1016/j.omto.2019.12.010

Figure Lengend Snippet: ST6GAL2 Expression Is Upregulated in FTC (A and B) The heatmaps and volcano plot show that ST6GAL2 expression was upregulated in FTC tissues. (C) According to the qPCR results, ST6GAL2 was overexpressed in FTC cells, especially in FTC238 cells. *p < 0.05; scale bars, 20 μm.

Article Snippet: FTC238 cells were purchased from the European Collection of Authenticated Cell Cultures (ECACC) (Shanghai, China).

Techniques: Expressing

Journal: Molecular Therapy Oncolytics

Article Title: Resveratrol Inhibits the Tumorigenesis of Follicular Thyroid Cancer via ST6GAL2-Regulated Activation of the Hippo Signaling Pathway

doi: 10.1016/j.omto.2019.12.010

Figure Lengend Snippet: ST6GAL2 Can Regulate the Tumorigenesis of FTC In Vitro and In Vivo (A and B) ST6GAL2 expression was downregulated and upregulated in the ST6GAL2 and si-ST6GAL2 groups of FTC133 and FTC238 cells, respectively. (C and D) ST6GAL2 expression regulated the proliferation of FTC cells, as evidenced by colony formation assays. (E–J) ST6GAL2 promoted development of FTC via enhancement of the migration, invasion, and angiogenesis capacity of FTC cells, whereas the opposite results were found in si-ST6GAL2-transfected FTC cells. (K and L) Tumor weight was measured after the tumors were removed. (M) Tumor growth curves were measured after injection of FTC238 cells transfected with ST6GAL2 or si-ST6GAL2. *p < 0.05; scale bars, 20 μm.

Article Snippet: FTC238 cells were purchased from the European Collection of Authenticated Cell Cultures (ECACC) (Shanghai, China).

Techniques: In Vitro, In Vivo, Expressing, Migration, Transfection, Injection

Journal: Molecular Therapy Oncolytics

Article Title: Resveratrol Inhibits the Tumorigenesis of Follicular Thyroid Cancer via ST6GAL2-Regulated Activation of the Hippo Signaling Pathway

doi: 10.1016/j.omto.2019.12.010

Figure Lengend Snippet: ST6GAL2 Expression Regulates the Hippo Signaling Pathway (A–C) Expression of the main protein components of the Hippo signaling pathway was investigated in FTC 238 cells by western blotting. Shown is Western blot analysis of nuclear YAP and TAZ expression in the indicated cells. The nuclear protein histone H3 was used as the nuclear protein marker. (D and E) IHC staining was performed to determine the expression levels of Hippo pathway signaling molecules in tumor tissues. (F) Representative immunofluorescence images of FTC238 cells immunostained with an anti-YAP antibody (red). *p < 0.05; scale bars, 20 μm.

Article Snippet: FTC238 cells were purchased from the European Collection of Authenticated Cell Cultures (ECACC) (Shanghai, China).

Techniques: Expressing, Western Blot, Marker, Immunohistochemistry, Immunofluorescence

Journal: Molecular Therapy Oncolytics

Article Title: Resveratrol Inhibits the Tumorigenesis of Follicular Thyroid Cancer via ST6GAL2-Regulated Activation of the Hippo Signaling Pathway

doi: 10.1016/j.omto.2019.12.010

Figure Lengend Snippet: Upregulation of ST6GAL2 Rescues Tumorigenesis of FTC238 Cells and Resuppresses Hippo Signaling Pathway Activity (A–F) ST6GAL2 knockdown cells were transfected with ST6GAL2 overexpression vectors, and the proliferation, migration, and invasion capacities of FTC cells were enhanced. (G and H) Western blotting was performed to determine the levels of Hippo signaling molecules in FTC cells. *p < 0.05; scale bars, 20 μm.

Article Snippet: FTC238 cells were purchased from the European Collection of Authenticated Cell Cultures (ECACC) (Shanghai, China).

Techniques: Activity Assay, Knockdown, Transfection, Over Expression, Migration, Western Blot

Journal: Molecular Therapy Oncolytics

Article Title: Resveratrol Inhibits the Tumorigenesis of Follicular Thyroid Cancer via ST6GAL2-Regulated Activation of the Hippo Signaling Pathway

doi: 10.1016/j.omto.2019.12.010

Figure Lengend Snippet: Res Reduces Tumorigenesis of FTC In Vitro and In Vivo (A and B) CCK-8 viability assays were performed to determine the effective concentration of Res. (C–J) Res suppressed tumorigenesis of FTC by reducing the proliferation, migration, invasion, and angiogenesis capacities of FTC cells. (K and L) Tumor weight was measured after the tumors were harvested. (M) Tumor growth curves were measured after injection of FTC238 cells. Each nude mouse was injected with NS or Res solution once every 2 days. *p < 0.05; scale bars, 20 μm.

Article Snippet: FTC238 cells were purchased from the European Collection of Authenticated Cell Cultures (ECACC) (Shanghai, China).

Techniques: In Vitro, In Vivo, CCK-8 Assay, Concentration Assay, Migration, Injection

Journal: Molecular Therapy Oncolytics

Article Title: Resveratrol Inhibits the Tumorigenesis of Follicular Thyroid Cancer via ST6GAL2-Regulated Activation of the Hippo Signaling Pathway

doi: 10.1016/j.omto.2019.12.010

Figure Lengend Snippet: Res Reduces ST6GAL2 Expression and Activates the Hippo Signaling Pathway in FTC Cells (A–D) The qPCR and western blotting results indicated that ST6GAL2 expression changes after Res treatment in FTC cells. (E–G) Expression of the main protein components of the Hippo signaling pathway in FTC238 cells was assessed by western blotting. Also shown is western blot analysis of nuclear YAP and TAZ expression in the indicated cells. The nuclear protein histone H3 was used as the nuclear protein marker. (H and I) IHC staining was performed to determine the expression levels of Hippo pathway signaling molecules in tumor tissues. *p < 0.05; scale bars, 20 μm.

Article Snippet: FTC238 cells were purchased from the European Collection of Authenticated Cell Cultures (ECACC) (Shanghai, China).

Techniques: Expressing, Western Blot, Marker, Immunohistochemistry

Journal: Molecular Medicine Reports

Article Title: X-irradiation induces acute and early term inflammatory responses in atherosclerosis-prone ApoE − / − mice and in endothelial cells

doi: 10.3892/mmr.2021.12038

Figure Lengend Snippet: Inflammatory response in endothelial cells at 24 h after irradiation. The response of various inflammatory markers at 24 h after 0.1 and 5 Gy irradiation is represented in (A-I) TICAE cells and (J-R) TIME cells, normalized to cell count. The Kruskal-Wallis test was used to analyse the data and the P-value was adjusted using the Benjamini-Hochberg method. Values represent the average ± SEM of 6 biological replicates. *P<0.05, **P<0.01 and ***P<0.001 vs. 0 Gy. p.i., post irradiation; GDF-15, growth differentiation factor-15; CXCL10, C-X-C motif chemokine ligand 10; ICAM-1, intercellular adhesion molecule-1; MCP-1, monocyte chemoattractant protein-1; uPAR, urokinase-type plasminogen activator receptor; PAI-1, plasminogen activator inhibitor-1; FGF-basic, basic fibroblast growth factor.

Article Snippet: In addition, TICAE cells, which are primary human coronary artery endothelial cells from the European Collection of Authenticated Cell Cultures (ECACC; HCAECs cat. no. 300-05a) that were transduced with retroviruses bearing the est2 gene, a yeast homologue of the human TERT protein ( , ), were used.

Techniques: Irradiation, Cell Counting

Journal: Molecular Medicine Reports

Article Title: X-irradiation induces acute and early term inflammatory responses in atherosclerosis-prone ApoE − / − mice and in endothelial cells

doi: 10.3892/mmr.2021.12038

Figure Lengend Snippet: Inflammatory response in endothelial cells at 72 h after irradiation. The response of various inflammatory markers at 72 h after 0.1 and 5 Gy irradiation is presented in (A-I) TICAE cells and (J-R) TIME cells. Data were normalized to cell count. The Kruskal-Wallis test was used to analyse the data and the P-value was adjusted using the Benjamini-Hochberg method. Values represent the average ± SEM of 6 biological replicates. *P<0.05, **P<0.01 and ***P<0.001 vs. 0 Gy. p.i., post irradiation; GDF-15, growth differentiation factor-15; CXCL10, C-X-C motif chemokine ligand 10; ICAM-1, intercellular adhesion molecule-1; MCP-1, monocyte chemoattractant protein-1; uPAR, urokinase-type plasminogen activator receptor; PAI-1, plasminogen activator inhibitor-1; FGF-basic, basic fibroblast growth factor.

Article Snippet: In addition, TICAE cells, which are primary human coronary artery endothelial cells from the European Collection of Authenticated Cell Cultures (ECACC; HCAECs cat. no. 300-05a) that were transduced with retroviruses bearing the est2 gene, a yeast homologue of the human TERT protein ( , ), were used.

Techniques: Irradiation, Cell Counting